Technical Data
C2069-43
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin)
Description:
a-catenin is a 102kD protein which is related to the cytoskeletal protein vinculin. Vinculin associates with both cell-cell and cell-extracellular matrix adherens-type junctions, and has been shown to interact with both a-actinin and actin. The homology between a-catenin and vinculin is localized to three major regions (amino-terminal, central, and carboxy-terminal portions) with the c-termini exhibiting the highest overall homology . Interestingly, it is the carboxy terminal domain of vinculin which is responsible for its self-association properties . The homology between the c-termini of catenin and vinculin led to speculation that a-catenin can self-associate and/or co-associate with vinculin thereby linking the cadherin/catenin complex to the cytoskeleton. However, recent studies indicate that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between a-actinin and a-catenin. In addition, the a-catenin protein has been found to posses both actin binding and bundling activities.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 2-5ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry ( formalin- xed, paraf n-embedded tissue): 2-10ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic.
Immunoprecipitation: 5ug
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas/breast tumor/heart

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3G59 (7A4)Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseMouse
Concentration:
0.5mg/ml
Immunogen:
Synthetic peptide derived from the C-terminus of mouse a-catenin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Also available diluted at 1ml. See C2069-43E.
Specificity:
Recognizes mouse a-catenin. Does not crossreact with the related beta- and gamma-catenin proteins. Species Crossreactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human broblasts, Xenopus cytosol, NIH 3T3 cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.