Technical Data
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin)
a-catenin is a 102kD protein which is related to the cytoskeletal protein vinculin. Vinculin associates with both cell-cell and cell-extracellular matrix adherens-type junctions, and has been shown to interact with both a-actinin and actin. The homology between a-catenin and vinculin is localized to three major regions (amino-terminal, central, and carboxy-terminal portions) with the c-termini exhibiting the highest overall homology . Interestingly, it is the carboxy terminal domain of vinculin which is responsible for its self-association properties . The homology between the c-termini of catenin and vinculin led to speculation that a-catenin can self-associate and/or co-associate with vinculin thereby linking the cadherin/catenin complex to the cytoskeleton. However, recent studies indicate that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between a-actinin and a-catenin. In addition, the a-catenin protein has been found to posses both actin binding and bundling activities.

Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 2-5ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry ( formalin- xed, paraf n-embedded tissue): 2-10ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic.
Immunoprecipitation: 5ug
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas/breast tumor/heart


Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k3G59 (7A4)Affinity Purified
100ug4C (-20C Glycerol)Blue IceMouseMouse
Synthetic peptide derived from the C-terminus of mouse a-catenin.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Also available diluted at 1ml. See C2069-43E.
Recognizes mouse a-catenin. Does not crossreact with the related beta- and gamma-catenin proteins. Species Crossreactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human broblasts, Xenopus cytosol, NIH 3T3 cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.