Technical Data
C2069-44J
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin)
Description:
Cellular Localization: Cell Membrane and Cytoplasmic

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide: EKQDETQTKIKRASQ conjugated to KLH, corresponding to amino acids 873-887 of Human alpha 1 Catenin.
Purity:
Purified
Form
0.01M PBS. pH 7.4
Specificity:
Recognizes alpha-E-catenin (102kD). Staining of the alpha-E-catenin band in immunoblotting is specifically inhibited with the alpha-E-catenin immunizing peptide (human, amino acids 873-887). Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen) and Xenopus laevis (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Knudsen KA et al. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol 130:67-77 (1995). Cowin P Unraveling the cytoplasmic interactions of the cadherin superfamily. Proc Natl Acad Sci U S A 91:10759-61 (1994). Ozawa M et al. Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule. Proc Natl Acad Sci U S A 87:4246-50 (1990). Nagafuchi A & Takeichi M Transmembrane control of cadherin-mediated cell adhesion: a 94 kDa protein functionally associated with a specific region of the cytoplasmic domain of E-cadherin. Cell Regul 1:37-44 (1989).