Technical Data
C2087-23T
Caspase 4, NT (Caspase-4, CASP 4, CASP4, CASP-4, Apoptotic Cysteine Protease Mih1/TX, ICEREL-II, ICE(rel)-II, ICH 2, Ich-2, ICH2 Protease, Mih1/TX, Protease ICH-2, Protease TX, TX)
Description:
Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller
p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimer’s disease and Ischemia and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml; Detects a band of ~34kD in Ramos cell lysates (predicted precursor MW 31kD).
Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Does not require antigen retrieval using heat treatment prior to staining.
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Spleen tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
16aa peptide from human Caspase-4 amino terminus.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes an epitope within the N-terminal region (NT) of human Caspase-4. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.