Technical Data
C2096-64E
CTNNB1 (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286)
Description:
Beta-catenin is an adherens junction protein, which mediates cell-cell adhesion, cell-cell communication and contributes to cytoskeletal integrity. It is similar to the product of the Drosophila segment polarity gene armadillo. Like armadillo, beta-catenin is part of the E cadherin multiprotein complex that regulates pattern formation at several stages of embryogenesis. In addition beta-catenin is involved in wound healing and tumor cell metastasis. Beta-catenin is part of the Wnt signaling cascade. It interacts with Tcf and Lef to activate gene transcription and regulates cell growth. Beta-catenin accumulation has been observed in various cancers, especially in colorectal tumors and hepatoblastoma. Beta-catenin-mediated transcription is down regulated when the protein binds to the tumor suppressor protein APC.

Applications:
Suitable for use in Flow Cytometry, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1EM-22Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant corresponding to human beta-catenin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.
Specificity:
Recognizes human beta-catenin. Species sequence homology: hamster and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.