Technical Data
CTNNB1 (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286)
Beta-catenin is an adherens junction protein, which mediates cell-cell adhesion, cell-cell communication and contributes to cytoskeletal integrity. It is similar to the product of the Drosophila segment polarity gene armadillo. Like armadillo, beta-catenin is part of the E cadherin multiprotein complex that regulates pattern formation at several stages of embryogenesis. In addition beta-catenin is involved in wound healing and tumor cell metastasis. Beta-catenin is part of the Wnt signaling cascade. It interacts with Tcf and Lef to activate gene transcription and regulates cell growth. Beta-catenin accumulation has been observed in various cancers, especially in colorectal tumors and hepatoblastoma. Beta-catenin-mediated transcription is down regulated when the protein binds to the tumor suppressor protein APC.

Suitable for use in Flow Cytometry, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1EM-22Affinity Purified
50ug-20CBlue IceHumanMouse
As reported
Recombinant corresponding to human beta-catenin.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 15mM sodium azide, pH 7.4.
Recognizes human beta-catenin. Species sequence homology: hamster and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.