Technical Data
Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal aspartyl peptidase, MGC2311)
Cathepsin D is a ubituitiously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43kD preprocathepsin D that is cleaved and glycosylated to form a 46kD procathepsin D and then further cleaved to produce 28kD and 15kD subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).

Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot 1:1000
Immunohistochemistry (Paraffin) 1:50
Flow Cytometry 1:25

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Synthetic peptide (KLH-coupled) corresponding to residues with the heavy chain subunit of human Cathepsin D.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Recognizes endogenous levels of human preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 49104914. (2) Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 1122411231. (3) Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167179. (4) Berchem, G. et al. (2002) Oncogene 21, 59515955. (5) Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 19. (6) Garcia, M. et al. (1996) Stem Cells 14, 642560. (7) Nogami, M. et al. (2000) Histochem. J. 32, 505508. (8) Nakanishi, H. (2003) Ageing Res. Rev. 2, 367381. (9) Callahan, L.M. et al. Neurobiol. Aging 19, S99105.