Technical Data
C2097-95
Cbl
Description:
Applications:
Suitable for use in Immunoblot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.5–2ug/ml detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with anti-Cbl (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 2ug of a previous lot immunoprecipitated Cbl from 1ug of a mouse 3T3 cell RIPA lysate.
Immunocytochemistry: 5–10ug/ml of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute.

Optimal dilution determined by the researcher.

Positive Control Included:
F4209: 3T3 lysate. Use 20ug per lane for minigels.


Storage and Stability:
Store at 4°C for short-term. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG0.T.12Affinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
GST fusion protein corresponding to residues 878–906 of human Cbl. This sequence is identical in mouse.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as liquid in PBS, 20% glycerol, 0.05% sodium azide.
Specificity:
Specific for Cbl. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Blake, T.J. et al. (1991) Oncogene 6, 653–657. 2. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473–482. 3. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294–307. 4. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323–8331. 5. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335–339. 6. Hunter, S. et al. (1999) J. Biol. Chem. 274, 2097–2106.