Technical Data
C2099-68
CCR 4 (C C chemokine receptor type 4, C C CKR 4, CC CKR 4, CCR 4, Chemokine (CC motif) receptor 4, chemokine C C motif receptor 4, ChemR13, CKR4, CMKBR 4, CMKBR4, HGCN 14099, K5 5, MGC88293)
Description:
Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

Applications:
Suitable for use in Western Blot, ELISA, Immunocytochemistry, Immunohistochemistry and Flow Cytometry/FACS. Other applications not tested.

Recommended Dilution:
ELISA: 1:500,000
FACS: 1-3ug/10e6cells/50-100ul
Immunocytochemistry: 1:500
Immunohistochemistry: 1:500
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse spleen cells

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseGoat
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide: DTTQDETVYNSYYFYESMPC, corresponding to amino acids 8-26 of mouse CCR4. Cellular Localization: Integral membrane protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide.
Specificity:
Recognizes mouse CCR4. Peptide sequence is < 50% identical to other mouse chemokine receptors in this region.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hoogewerf, A., et al., Biochem. Biophys. Res. Commun. 218: 337-43 (1996).