Technical Data
C2099-69K
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, CD195, CHEMR13, CKR5, CKR-5, CMKBR5, HIV-1 Fusion Coreceptor, IDDM22)
Description:
C-C Chemokine Receptor type 5 (CCR5) is a G-protein coupled receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES. CCR5 and its ligands may also play important roles in the control of granulocytic lineage proliferation or differentiation as well as pathogenesis of viruses such as HIV.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry: 0.5-1ug/ml for formalin fixed paraffin tissues. Heat mediated antigen retrieval is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to aa19-34, PCQKINVKQIAARLLP from the N-terminal of human CCR5 conjugated to BSA. Species sequence homology: rat and mouse
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes human CCR5 at ~41kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.