Technical Data
C2099-99H
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, AM4-7, CD195, CHEMR13, CKR5, CMKBR5, FLJ78003, HIV-1 Fusion Coreceptor, IDDM22)
Description:
CCR5 is a cell surface antigen of ~40kD, also known as CD195. This antibody has been shown to exclusively recognise CD195 which is non-phosphorylated at Ser337. Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes. CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation. Recent studies suggest that phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul
Immunofluorescence: 1:25-1:50
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B940Affinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-Terminal region of non-Phosphorylated human CD195 conjugated to BSA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CCR5 at ~40kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.