Technical Data
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, AM4-7, CD195, CHEMR13, CKR5, CMKBR5, FLJ78003, HIV-1 Fusion Coreceptor, IDDM22)
CCR5 is a cell surface antigen of ~40kD, also known as CD195. This antibody has been shown to exclusively recognise CD195 which is non-phosphorylated at Ser337. Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes. CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation. Recent studies suggest that phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases.

Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul
Immunofluorescence: 1:25-1:50
Optimal dilutions to be determined by the researcher.

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG110B940Affinity Purified
100ug4C/-20C (No Glycerol)Blue IceHumanMouse
Synthetic peptide corresponding to the C-Terminal region of non-Phosphorylated human CD195 conjugated to BSA.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes human CCR5 at ~40kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.