Technical Data
C2099-99H
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, AM4-7, CD195, CHEMR13, CKR5, CMKBR5, FLJ78003, HIV-1 Fusion Coreceptor, IDDM22)
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Technical Data |
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C2099-99H |
CCR5 (Chemokine (C-C motif) Receptor 5, C-C Chemokine Receptor Type 5, CCCKR5, CC-CKR-5, C-C CKR-5, CCR-5, AM4-7, CD195, CHEMR13, CKR5, CMKBR5, FLJ78003, HIV-1 Fusion Coreceptor, IDDM22) |
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Description: CCR5 is a cell surface antigen of ~40kD, also known as CD195. This antibody has been shown to exclusively recognise CD195 which is non-phosphorylated at Ser337. Human CD195 is a member of the G protein coupled superfamily of receptors, which acts as a receptor for a number of chemokines including RANTES and also serves as a co-receptor for the entry of HIV into cells. It plays a key role in regulating the activation and migration of leukocytes. CD195 is expressed by a subset of T lymphocytes and by monocytes. CD195 is rapidly phosphorylated within the C-terminal domain upon ligand stimulation. Recent studies suggest that phosphorylation at Ser337 is mediated by Protein kinase C, whereas phosphorylation at Ser349 is mediated by GPCR kinases. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul Immunofluorescence: 1:25-1:50 Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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