		Technical Data
C2100-20A	CCR11 (Chemokine Receptor 11, CKRB)

Description:
Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR11 in particular is expressed in the heart, lung, and small intestine. It binds members of the monocyte chemoattractant family including MCP-1, MCP-2, and MCP-4.

Application(s):
Suitable for use in Western Blot, ELISA, Immunohistochemistry (Formalin-fixed paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:75,000
Immunocytochemistry: 1:500
Immunohistochemistry (Paraffin): 1:250
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human heart, lung or small intestine.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~2mg/ml

Immunogen:

Synthetic peptide: EQNQSTDYYYEENEMNGTYDYSQYELIC, corresponding to amino acids 4-31 of Human CCR11.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide.

Specificity:

Recognizes human CCR11. Species Crossreactivity: Mouse. Peptide sequence is < 50% identical to other human chemokine receptors in this region.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Schweickart VL et al. CCR11 is a functional receptor for the monocyte chemoattractant protein family of chemokines. J Biol Chem 275 :9550-6 (2000). PubMed: 10734104.