Technical Data
C2102
CCR10 (CC Chemokine receptor 10, CC-CCR-10, G protein-coupled receptor 2, GRP2)
Description:
Recently, a function has been assigned to a previously identified orphan chemokine receptor, GPR-2. This receptor, also called CCR10, binds with monocyte chemoattractant protein (MCP)-1, MCP-3 and MIP-1 beta. CCR10 is expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, T cells as well as in skin-derived Langerhans cells. CCR10 may play a role in both skin homeostasis as well as a potential role in inflammatory responses.

Applications:
Suitable for use Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml In A-375, ~50kD band is observed.
Optimal dilutions to be determined by the researcher.

Positive Control: A-375 or MCF-7

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Peptide corresponding to amino acids 350-362 of human CCR10.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 0.2% gelatin.
Specificity:
Recognizes CCR10. Species Crossreactivity: Human. Predicted to crossreact with mouse. In this region, mouse and human amino acid sequences are 100% identical.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonini JA, et al. DNA Cell Biol 16(10): 1249-56 (1997). 2. Bonini JA, et al.. DNA Cell Biol 16(9): 1023-30 (1997). 3. Jarmin DI, et al. J Immunol 164(7): 3460-4 (2000). 4. Homey B, et al.. J Immunol 164(7): 3465-70 (2000). 5. Wang W, et al.. J Biol Chem 275(29): 22313-23 (2000).