		Technical Data
C2252-03E	CD1c

Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Use 10ul to label 1x10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized Biozzi mice were fused with cells of the NS1 mouse myeloma cell line.

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo
I1904-66A: IgG, F(ab')2 (FITC) Pab Rb xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H648	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Thymus cells.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide.

Specificity:

3H648 recognizes a polypeptide (CD1c) antigen of 43kD which is associated with the 12kD beta 2 microglobulin present on thymocytes, a subpopulation of B-lymphocytes early during ontogeny, Langerhans cells and dendritic cells. It may be useful for studies of calcium flux induced by CD1c.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Amiot, M. et al. (1986) J. Immunol. 136: 1752-1758. 2. Amiot, M. et al. (1987) Blood. 70 (3): 676-685. 3. Amiot, M. et al. (1988) Immunogenetics 27: 187-195. 4. Baumsell, L. et al. (1989) Oxford University Press, Oxford. 251 - 254. 5. Theodorou, I.D.et al. (1990) J. Immunol. 144 (7): 2518 - 2523.