Technical Data
C2253-07R
CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor) (Biotin)
Description:
CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggerring of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukemias of T cell origin.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry
Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen, Paraffin (see ref 2)
Flow Cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H657Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
Activated rat T helper cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 1% BSA. Labeled with Biotin.
Specificity:
Recognizes the rat CD2 cell surface antigen, a 50-54kD glycoprotein expressed by thymocytes and mature T cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Williams, A.F. et al. (1987). J. Exp. Med. 165: 368-380. 2. Whiteland, J.L. et al . (1995). Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43: 313-320. 3.Barclay A.N. Immunology 42:593-600 (1981).