		Technical Data
C2254-38B	CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)

Description:
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100; Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections. Does not stain formalin fixed paraffin embedded sections.
Optimal working dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized SD rats were fused with cells of the NSO mouse myeloma cell line.

Recommended Secondary Antibody:
I1903-13C: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)

Recommended Negative Control:
I1904-82D1: Rat IgG2a Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H698	Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~1mg/ml

Immunogen:

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes the mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells. For optimal staining incubations should be performed at room temperature.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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