Technical Data
CD4 (Tac Antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Biotin)
Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the “helper/inducer” subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells and 25% of peripheral blood lymphocytes (1-3). CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Frozen sections (acetone fixed)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ug4°C (-20°C Glycerol)Blue IceFelineMouse
Feline CD4
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Recognizes feline CD4, Mr 65kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ackley, C.D., et al., Tissue Antigens 35: 92-98 (1990). 2. Ackley, C.D., et al., J. Virol. 64: 5652-5655 (1990). 3. Dean, G.A., et al., Vet. Immunol. Immunopathol. 28: 327-335 (1991).