Technical Data
C2255-13F
CD4 (Tac Antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Biotin)
Description:
Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the “helper/inducer” subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells and 25% of peripheral blood lymphocytes (1-3). CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Frozen sections (acetone fixed)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5K113Purified
SizeStorageShippingSourceHost
500ug4°C (-20°C Glycerol)Blue IceFelineMouse
Concentration:
~0.5mg/ml
Immunogen:
Feline CD4
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes feline CD4, Mr 65kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ackley, C.D., et al., Tissue Antigens 35: 92-98 (1990). 2. Ackley, C.D., et al., J. Virol. 64: 5652-5655 (1990). 3. Dean, G.A., et al., Vet. Immunol. Immunopathol. 28: 327-335 (1991).