Technical Data
C2255-13F
CD4 (Tac Antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Biotin)
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Technical Data |
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C2255-13F |
CD4 (Tac Antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Biotin) |
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Description: Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the “helper/inducer” subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells and 25% of peripheral blood lymphocytes (1-3). CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Immunohistochemistry: Frozen sections (acetone fixed) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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