		Technical Data
C2259-36	CD8 (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha, Suppressor/Cytotoxic T cell)

Description:
The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation (3). The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p561ck)(4).

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen tissue and formalin-fixed paraffin embedded tissue sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	2Q1751	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice	Rat	Mouse

Concentration:

~1mg/ml

Immunogen:

Rat thymocyte membrane glycoproteins.

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a liquid in PBS, pH 7.2, 10mM sodium azide.

Specificity:

Recognizes rat CD8 alpha antigen present on a subset of T-cells that has suppressor and cytotoxic activities. 90% of thymocytes and NK cells are labelled with CD8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Brideau R. J., et al. Eur. J. Immunol. 10, 609–615 (1980). 2. Williams A. F., Galfre G & Milstein C, Cell 12, 663–673 (1977). 3. Dallman M.J. et al. Eur. J. Immunol. 12, 511–518 (1982). 4. Cantrell D. A. et al. Immunology, 45, 97–103 (1982). 5. Mason D. W. Transplantation 32, 222–226 (1981). 6. Johnson P & Williams A. F. Nature 323, 74 (1986). 7. Whiteland J. L. Nicholls S.M., Shimeld C. et al. J. Histochem 43(3), 313–320 (1995)