Technical Data
C2261-40R
CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17)
Description:
CD10 is zinc-dependent metalloprotease enzyme that plays a role in the destruction of opiod peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (1). CD10 is a cell-surface glycoprotein expressed on most acute lymphoblastic leukemias and certain other
immature lymphoid malignancies and on normal lymphoid progenitors. The latter are either uncommitted to B- or T-cell lineage or committed to only the earliest stages of B- or T-
lymphocyte maturation.CD10 is not restricted to leukemic cells, however, and is found on a variety of normal tissues. CD10 is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium (2). The proteolysis of beta- amyloid (Abeta) requires CD10, an enzyme that has been shown as reduced in Alzheimer's disease (AD).The defect of CD10 appears to correlate with Abeta deposition but not with degeneration and dementia (3).

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:150
Western Blot: 1:5000-1:10000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H324Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on the N-terminus of human CD10.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human CD10. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 2. Shipp MA, et al. Natl Acad Sci 85(13):4819-23, 1988 3. Russo R, et al. FEBS Lett 579(27) :6027-30, 2005