Technical Data
C2262-36H
CD11b (Mac-1, alphaM integrin) (CT)
Description:
Integrin alpha-M (ITAM, ITGAM, CD11b, MAC-1 alpha subunit, CR3 alpha chain) is the alpha subunit of ITAM/beta-2 complex (CD11b/CD18, Mac-1), a leukocyte adhesion heterodimeric glycoprotein. ITAM belongs to integrin alpha chain family and it is predominately presented in human myeloid cells, NK1 cells, monocytes, and granulocytes. ITAM/beta-2 complex is a receptor for fibrinogen (binding to P1 and P1 peptides of gamma chain), factor X, and ICAM1. ITAM/beta-2 is implicated in adhesive interactions of monocytes, macrophages, and granulocytes. It is also linked to mediation of complement-coated particle uptake.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry (Paraffin): 1:100-1:250
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:20
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9E194Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to the C-terminus of human CD11b/ITAM protein.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% Glycerol.
Specificity:
Recognizes human CD11b, Integrin alpha-M at ~170kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Corbi AL et al. J. Biol. Chem. 263(25):12403-12411, 1998. 2. Barden A et al. Clin Sci (Lond). 92(1):37-44m 1997.