Technical Data
C2262-39L
CD11B (Integrin alpha-M, CD11 Antigen-like Family Member B, CR-3 alpha Chain, Cell Surface Glycoprotein MAC-1 Subunit alpha, Leukocyte Adhesion Receptor MO1, Neutrophil Adherence Receptor, ITGAM, CR3A)
Description:
The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170kD type I transmembrane glycoprotein also known as aM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin b2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the alpha chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kCBRM1/5Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human CD11b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Diamond, M., et al., 1993. J. Cell Biol. 120:545. 2. Bryn, T., et al., 2006. J. Immunol. 176: 7361. 3. Sithu, S.D., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200