Technical Data
C2262-52D
CD11b (Mac-1, alphaM integrin, Cr3, MO-1, C3bi Receptor) (PE)
Description:
Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1). The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, in vitro and in vivo Blocking. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug stains 1x10e6 cells
Immunohistochemistry: Acetone- xed frozen tissue sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
TypeIsotypeCloneGrade
MabIgG2b1.BB.633Purified
SizeStorageShippingSourceHost
100ug4°C Do not freezeBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
C57BL/10 mouse spleen cells enriched for T lymphocytes.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 2mM EDTA, 1% BSA, 0.1% sodium azide. Labeled with PE.
Specificity:
Recognizes the Mr170kD alpha-m subunit of Mac-1, the mouse macrophage-granulocyte speci c antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Springer, T. et al. (1978) Eur. J. Immunol. 8:539. 2. Vignali, D. A. et al. (1990) J. Immunol. 144:4030 3. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320 4. Jutila. M. A. et al. (1988) J. Immunol. 18:1819