Technical Data
CD16 (Fc gamma RIII alpha, NK cells)
CD16 is the low affinity Fc gamma III present on Large Granular Lymphocytes (LGL), NK cells, granulocytes and a macrophage subpopulation. It is a late myeloid differentiation antigen and expressed on Chronic Myeloid Leukemia cells. The antibodies LGL/1 and LGL/3 show a polymorphism for granulocytes. LGL/1 reacts with granulocytes of a proportion of the human population and LGL/3 reacts with the granulocytes of another proportion of the population. The nature of this polymorphism of granulocytes has not yet been revealed.

Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a5B11Affinity Purified
100ug-20CBlue IceMouse
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Recognizes CD16 antigen. Species Crossreactivity: human, rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.