Technical Data
C2269
CD18 (Leukocyte Function Associated Antigen, LFA-1 beta2)
Description:
LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation.

Hybridoma:
Formed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Adhesion Blocking. Other applications not tested.

Working Dilution:
Flow Cytometry: 1:100-1:200 (0.5-1ug will label about 10e6 cells)

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (25–50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18.S.303Affinity Purified
SizeStorageShippingSourceHost
200ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat FTL-43 cells
Purity:
Purified by affinity chromatography.
Form
Liquid in PBS, 0.02% sodium azide
Specificity:
Recognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts and blocks binding of rat lymphocytes to purified rat ICAM-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tamatani, T. et al., Eur. J. Immunol., 21:627 - 633 (1991)
2. Tamatani, T. et al., Eur. J. Immunol., 21:855 - 858 (1991)