Technical Data
C2269-60
CD19
Description:
CD19 is a 95kD type I transmembrane glycoprotein which is restricted B cell antigen. CD19 antigen is expressed on normal and neoplastic B cells and also in some the bone marrow cells. CD19 expression by B progenitor cells is presumably at late pro-B or early pre-B stages around the time of Ig heavy chain rearrangement. Expression persists during all stages of B cell maturation and is lost on terminal differentiation to plasma cells. CD19 antigen is also found on the follicular dendritic cells and the early cells of myelomonocytic lineage but not on normal T cells, NK cells, monocytes, granulocytes, erythrocytes and platelets. In normal peripheral blood, 8-20% of lymphocytes express CD19 antigen. CD19 antigen plays a role in regulating B cell proliferation.

Applications:
Suitable for use in Indirect Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.015ug labels 10e6 cells or 100ul peripheral blood
Immunohistochemistry: Acetone-fixed frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k5F22Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CD19
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human CD19. Reacts with normal human peripheral blood lymphocytes (11%). Does not react with peripheral blood monocytes and granulocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schlossman, S., L.Bloumsell, W.Gilks, et al., eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. P:491 507 2004 Oxford University Press, New York.