		Technical Data
C2270-08C	CD20 (CT) (MS4A1, B1, S7, Bp35, MS4A2, LEU-16, MGC3969, membrane-spanning 4-domains, subfamily A, member 1, B-lymphocyte cell-surface antigen B1)

Description:
CD20, also known as B1 and MS4A1, is a human B lymphocyte surface molecule expressed on both normal and malignant cells that functions as a receptor during B cell activation. It is also involved in the regulation of cell progression in B lymphocyrtes as either a component or a regulator of intracellular calcium levels (1-2). CD20 is phosphorylated on serines primarily and threonines but not tyrosines. Monoclonal antibodies against CD20 are used to treat B-cell non-Hodgkin’s lymphoma (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:1000-5000
Immunohistochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	6k103	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

A synthetic peptide corresponding to residues in the C-term of human CD20. Molecular Weight: ~35kD

Purity:

Supernatant

Form

Supplied as a liquid in Tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.05% BSA, 0.01% sodium azide.

Specificity:

Recognizes human CD20.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tedder, T. F.; Klejman, G.; Schlossman, S. F.; Saito, H.: J. Immun. 142: 2560-2568, 1989. 2. Tedder, T. F.; Streuli, M.; Schlossman, S. F.; Saito, H.:Proc. Nat. Acad. Sci. 85: 208-212, 1988. 3. Fischer M, Behr T, Grunwald F, Knapp WH, Trumper L, von Schilling C; German Society for Hematology and Oncology, Nuklearmedizin. 2004 Oct;43(5):171-6.