Technical Data
C2270-08C
CD20 (CT) (MS4A1, B1, S7, Bp35, MS4A2, LEU-16, MGC3969, membrane-spanning 4-domains, subfamily A, member 1, B-lymphocyte cell-surface antigen B1)
Description:
CD20, also known as B1 and MS4A1, is a human B lymphocyte surface molecule expressed on both normal and malignant cells that functions as a receptor during B cell activation. It is also involved in the regulation of cell progression in B lymphocyrtes as either a component or a regulator of intracellular calcium levels (1-2). CD20 is phosphorylated on serines primarily and threonines but not tyrosines. Monoclonal antibodies against CD20 are used to treat B-cell non-Hodgkin’s lymphoma (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:1000-5000
Immunohistochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k103Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues in the C-term of human CD20. Molecular Weight: ~35kD
Purity:
Supernatant
Form
Supplied as a liquid in Tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.05% BSA, 0.01% sodium azide.
Specificity:
Recognizes human CD20.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tedder, T. F.; Klejman, G.; Schlossman, S. F.; Saito, H.: J. Immun. 142: 2560-2568, 1989. 2. Tedder, T. F.; Streuli, M.; Schlossman, S. F.; Saito, H.:Proc. Nat. Acad. Sci. 85: 208-212, 1988. 3. Fischer M, Behr T, Grunwald F, Knapp WH, Trumper L, von Schilling C; German Society for Hematology and Oncology, Nuklearmedizin. 2004 Oct;43(5):171-6.