Technical Data
CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)
Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4).

Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Flow Cytometry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Flow Cytometry Protocol:
1. Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM).
2. Five x 10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of aC2275-83A at 5ug/ml.
3. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a,k7H136Affinity Purified
100ug-20CBlue IceHumanMouse
Stimulated PBMC
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
Recognizes epitope B of the human IL-2 alpha receptor of about 55kD. Does not block IL-2 binding to its receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. L.A. Rubin, et al, (1985) Hybidoma 4: 91-102. 2) B. Boutin, et al, (1989) Neuropediatrics 20: 202-206.3) T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911. (4) T.A. Waldmann, et al, (1995) Blood 86: 4063-4075. (5) U.S. Patent No. 4,707,443. (6) Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 403-406