Technical Data
CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2)
CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Recombinant protein including aa 238-546 of human CDT1. Cellular Localization: Nucleus. Epitope: C-terminal half.
Supplied as liquid, 0.05% sodium azide.
Recognizes human Cdt1. Species crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wohlschlegel, B.T. et al (2000). Science: 290: 2309-2312. 2. Liu, E. et al. (2004). J. Biol. Sci. 279(17): 17283-17288. 3. Sansam, C.L. et al. (2006) Genes and Development: 20:3117-3129.