Technical Data
C2300-02
CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2)
Description:
Geminin is a protein that inhibits DNA replication by preventing the loading of MCM complex on chromatin. Human CDT1 is a 65kDa protein that contains 546 amino acids and coimmunoprecipitates with Geminin. Inhibition of DNA replication by Geminin in cell-free cDNA replication extracts can be reversed by the addition of excess CDT1. In the normal cell cycle, CDT1 is present only in G1 and S phases, whereas Geminin is present in S and G2 phases.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2500
Immunoprecipitation: 2-5ug/mg lysate
Immunohistochemistry (paraffin): 1:100-1:500. Requires heating in citrate buffer, pH 6 for antigen retrieval.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide representing the C-terminus of the human Chromatin Liscensing & DNA Factor 1 protein (residues 500-546) according to NP_112190.1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate buffer, pH7-8, containing 0.09% sodium azide
Specificity:
Recognizes human CDT1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.