Technical Data
CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
CD44 is the receptor for hyaluronan. The interaction between CD44 and hyaluronan may mediate binding of cells to basement membranes. Several CD44 isoforms or variants arise from alternative splicing of mRNA transcribed from a single gene located on chromosome 11. The alternatively spliced mRNAs are translated into a family of protein isoforms which contain inserts in their cytoplasmic domains of varying lengths. Most cell types express the standard CD44, while the expression of the isoforms resulting from the alternative splicing is of limited distribution. CD44 isoforms resulting from alternative splicing have been detected in many human tumors; therefore, the identification of CD44 isoforms has utility in cancer studies.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Immunohistochemistry (acetone fixed frozen sections or cell smears): 1ug/ml APAAP or PAP procedure or the three stage immunoperoxidase technique.
Immunohistochemistry (paraffin sections): 2ug/ml; after 2x microwave treatment for 5 minutes.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG16D27Highly Purified
100ug-20CBlue IceHumanMouse
Recombinant human CD44.
Purified 95% (SDS-PAGE).
Supplied as a liquid in PBS, pH 7.2-7.4.
Recognizes human CD44. Does not distinguish between CD44 splice variants (exons v3-v10).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Expression of CD44 splice variants in normal, dysplastic, and neoplastic cervical epithelium: S.M. Woerner, et al.; Clin. Cancer Res. 1, 1125 (1995)