Technical Data
C2399-07G2
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
Description:
CD45 or Leukocyte Common Antigen (LCA/T200) family is comprised of at least four isoforms (CD45RA, CD45RB, CD45RC, and CD45RO) of membrane glycoproteins with MW of 180-220kD. It consists of a large, heavily glycosylated, amino-terminal extracellular domain of 400-550 amino acids, a single transmembrane region and a large carboxy-terminal cytoplasmic domain of 700 amino acids. CD45/LCA is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of CD45 proteins has phosphatase activity and is involved in regulation of transmembrane signals, functioning as a leukocyte membrane receptor capable of transducing signals via its phosphotyrosine phosphatase domains.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): 1:500-1:1000
Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA, pH8.0 is recommmeded.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Recommended Positive Control:
Human Tonsil

Recommended Negative Control:
I1904-83P1: IgG2a Murine Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG2a10B1611Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human T lymphocytes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human CD45. Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Species crossreactivity: equine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dalchau, R. et al. (1980) Eur. J. Immunol. 10: 737-744. 2. Quenby, S et al. (1999) Human Reprod. 14(9):2386-2391. 4. Hauser, P.V. et al. (2010) Am J Pathol. 177: 2011-21. 5. Mallam, E. et al. (2010) Mult Scler. 16: 909-18.6. Marrinucci, D. et al. (2010) J Oncol. 2010: 861341.7. Kazane, S.A. et al. (2012) Proc Natl Acad Sci U S A. 109: 3731-6. 8.. Carrade, D.D. et al. (2012) Cell Medicine. 4: 1-11.