		Technical Data
C2399-08V	CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Azide Free)

Description:
CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 while those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunopreciptiation. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:100-1:200. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
I1904-65V: IgG, F(ab’)2 (FITC) Pab RbxMo
I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab RbxMo
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
F(ab')2 rabbit anti mouse IgG: RPE

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

IHC Positive Control:
Human tonsil

Negative Control:
Mouse IgG2a Negative Control, azide free

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	3H1378	Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human T lymphocytes

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

Specificity:

Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher levels of expression on lymphocytes than on granulocytes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dalchau, R., Kirkley, J., Fabre, J.W.: Monoclonal antibody to a human leucocyte specific membrane glycoprotein probably homologous to the leucocyte common antigen of the rat. European Journal of Immunology 10: 737-744 (1980).