Technical Data
C2400-67
CD45RO (Biotin)
Description:
The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive in ltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes. When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore, anti-CD45RO may be a marker for memory T-cells.

Applications:
Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues; frozen sections; cell suspensions.
Flow Cytometry: 1ug to stain 1x10e6 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a1.BB.437Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
IL-2 dependent T-cell line CA1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.01M PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes the low MW (Mr 180kD) isoform of the human leukocyte common antigen (LCA) which is part of a family of protein tryosine phosphatases.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Terry, L.A., et al., Immunology 64: 331 (1988). 2. Smith, S.H., et al., Immunology 58: 63 (1986). 3. Norton, A.J., et al., J. Clinc. Pathol. 39: 399 (1986).