Technical Data
C2414-94B
CD62E (LECAM, E-Selectin, ELAM1, Endothelial Leukocyte Adhesion Molecule)
Description:
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion
receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that
is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation,
Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Flow Cytometry: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b4A24Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
1mg/ml
Immunogen:
CD62E (LECAM, E-Selectin, ELAM1, Endothelial Leukocyte Adhesion Molecule)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes a conformational epitope in the CR2 region of mouse CD62E. Partially inhibits leukocyte adhesion to mouse activated endothelial cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hallmann R, Zimmermann U, Sorokin L, Needham L, von der Mark K. Scand J Rheumatol Suppl 101: 107-109 (1995). 2. Hammel M, Weitz-Schmidt G, Krause A, Moll T, Vestweber D, Zerwes HG, Hallmann R. Exp Cell Res 269: 266-274 (2001).