Technical Data
C2425-01C
CD70 (Tnfsf7, CD27 Ligand)
Description:
CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells.

Source:
Tissue culture supernatant

Applications:
Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 8-25ug/ml labels immersion fixed human peripheral blood mononuclear cells
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b7H76Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to human CD27 Ligand expressed in NS0 (Gln45-Pro193, Accession #AAA36175).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human CD27 Ligand. In Western Blot, shows no crossreactivity with recombinant mouse CD27 Ligand, recombinant human CD30 Ligand or recombinant human CD40 Ligand.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.