Technical Data
C2439-60G1
C5aR (C5a Anaphylatoxin Chemotactic Receptor, C5a-R, CD88, C5ar1, C5r1)
Description:
The rat anaphylatoxin C5a, a 77aa glycopolypeptide generated by proteolytic cleavage of C5, is one of the most potent inflammatory chemo attractants and interacts with two C5a receptors, C5aR and C5L2. Most of the C5a effects are mediated through C5aR. C5aR is known to be crucial in the initiation of acute inflammatory responses. C5a receptor is a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45kD C5a receptor is expressed by leukocytes such as neutrophils, eosinophils, basophils, monocytes, dendritic cells and mast cells. C5aR can be expressed on both immune and non-immune cells. The latter, include vascular endothelial cells, cardiomyocytes and bronchial epithelial cells. Signaling of C5aR involves intracellular calcium mobilization and activation of among others protein kinase B (PKB) and protein phospholipase D (PLD) pathways. High C5aR expression levels have been associated with the pathogenesis of many inflammatory conditions, autoimmune and neurodegenerative diseases like rheumatoid arthritis, multiple sclerosis, atherosclerosis and inflammatory bowel disease. In vivo blockade of C5aR greatly reduces inflammatory injury. Using immunohistology there is no evidence for non-myeloid expression in healthy control rats. However, under inflammatory situations, the C5aR was found to be upregulated in various organs and tissues including the liver.

Applications:
Suitable for use in Flow Cytometry, Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2x10e5 cells were incubated with mAb at 3-5ug/ml, all incubations on ice
Immunohistochemistry (Frozen): 7um frozen paraformaldehyde fixed sections were blocked with serum and incubated with mAb at 5ug/ml
Western Blot: cell homogenate was separated on 10% SDS-PAGE and blotted on nitrocellulose. After blocking blot was incubated with mAb (1:500-1:1000)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113A28Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
RBH-2H3 cells transfected with rat C5aR gene
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes the amino-terminal domain Ex1 of the rat C5aR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schlaf, G et al; Differential expression of the C5a receptor on the main cell types of rat liver as demonstrated with a novel monoclonal antibody and by C5a anaphylatoxin-induced Ca2+ release. Lab Invest 1999, 79: 1287. 2. Rothermel, E et al; Analysis of the tissue distribution of the rat C5a receptor and inhibition of C5a- mediated effects through the use of two MoAbs. Scand J Immunol 2000, 52: 401. 3. Schieferdecker, H et al; Induction of functional anaphylatoxin C5a receptors on hepatocytes by in vivo treatment of rats with IL-6. J Immunol 2000, 164: 5453. 4. Griffin, R et al; Complement induction in spinal cord microglia results in anaphylatoxin C5a- mediated pain hypersensitivity. J. Neurosc. 2007, 27: 8699. 5. Woodruff, T et al; The complement factor C5a contributes to pathology in a rat model of amyoptrophic lateral sclerosis. J Immunol 2008, 181: 8727. 6. Crane, J et al; The C5a anaphylatoxin receptor CD88 is expressed in presynaptic terminals of hippocampal mossy fibres. J Neuroinflamation 2009, 6: 34.