Technical Data
Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains.

Suitable for use in Flow Cytometry and Immunohistochemistry Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Suitable for use on unfixed frozen tissue sections of Thy.1 antigen, notably in studies of neuroblastoma, leukemia phenotyping and carcinoma of the breast and Studies of renal corticol fibroblasts in progressive renal disease. Epitope is resistant to trypsin treatment used to release adherent cells from surfaces. Not tested on fixed tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG12Q1469Highly Purified
100Tests-20CBlue IceHumanMouse
Not Determined
Purified by ammonium sulfate precipitation followed by ion exchange chromatography
Supplied as a liquid in PBS, 0.1% BSA. 10mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Recognizes human CD90 (Thy-1) molecule, a heavily glycosylated membrane bound protein of 18kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Altman, A.M. et al., (2008) Biomaterials. 29:1431-142. 1. Clayton, A. et al.J Am Soc Nephrol 8, 604615 (1997)2. Dalchau, R. et al. Immunol. Neurology & Therap. Appl. pp18595 Ed. Reif & Schlesinger, (1989) 3. McKenzie, J. L. & Fabre, J. W. Brain Res. 230, 307316 (1981)