Technical Data
C2443-86A
CD98
Description:
CD98 (also known as fusion regulatory protein-1, FRP-1) is widely distributed on hemopoietic and non-hemopoietic cells. The levels of CD98 expression is very high on most cell lines but is low on platelets and granulocytes. CD 98 expression is increased on lymphocytes after their activation. Erythrocytes do not express CD98. CD98 is a disulfate-linked 125kD heterodimeric membrane glycoprotein that is composed of an 80-90kD glycosylated heavy chain and a 40kD nonglycosylated light chain. It may be involved in the regulation of cellular activation.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagent:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1783Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
HOON pre-B leukemia cell line
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes the heavy chain of the human CD98 molecule. Binds to all cell lines in culture and to activated T-cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Letarte, M., et al., Biochem. Cell. Biol. 64: 1160-1169 (1986).