Technical Data
C2446-05B
CD104 (Integrin beta4) (FITC)
Description:
Human beta4 integrin, also known as CD104, is a 205kD glycoprotein. It associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:10; Use 10ul to label 10e6 cells in 100ul
Immunohistochemistry: Frozen
Optimal working dilutions to be determined by researcher.

Recommended Negative Control:
I1904-78R3: IgG1 Murine Negative Control (FITC)

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H1804Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified alpha6 beta4 integrin from A431 cells
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with FITC.
Specificity:
Recognizes an extracellular epitope on the human CD104 molecule.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kennel, S.J., et al., Second generation monoclonal antibodies to the human integrin alpah6 beta4. Hybridoma 9: 243-254 (1990). 2. Barbaro, V. et al., Mol. Vis 15:2084-93 (2009)