Technical Data
CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (BIotin)
CD106 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for CD106 is VLA-4/CD49d. In addition to CD106, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of CD106 and VLA-4 is able to provide a second signal for T cell stimulation, such as that seen in transplantation. The monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, CD106 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106 exists as an integral membrane protein.

Suitable for use in Flow Cytometry, Immunoflourescence Immunohistochemistry, Immunoprecipitation, Blockage of lymphocyte adhesion in Whitlock-Witte long-term bone marrow cultures and Inhibition of lymphopoiesis in Whitlock-Witte cultures. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1ug labels 10e6 cells
Immunoflourescence: Used for the identification of VCAM-1+ cells.
Immunohistochemistry: Used for the identification of VCAM-1+ cells.
Immunoprecipitation: Immunoprecipitates a peptide that gives a single band on SDS-PAGE gels at ~100kD under reducing conditions and ~92kD under non-reducing conditions
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ug-20CBlue IceMouseRat
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.
Recognizes mouse CD106/VCAM-1 (~Mr 100kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miyake, K., I.L. Weissman, J.S. Greenberger and P. W. Kincade. 1991. J. Exp. Med. 173:599. 2. Miyake, K., et al.. 1991. J. Cell. Biol. 114(3):557. 3. Schlegel, P.G., et al. 1995. J. Immunol. 155:3856. 4. Bergese, S., et al. 1995. Am J. Phathol. 146:989. 5. Orosz, C.G., et al. 1993. Transplantation 56:453.