Technical Data
CD134 Ligand, Blocking (CD134L, OX40L, CD252)
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional Assays. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/ml labels 5x10e5 cells.
Functional Assays: Blocks binding of CD134L to CD134.
Optimal dilutions to be determined by the researcher.

Flow Cytometry Protocol:
1. Rat TART-gp34 cells (5x10e5) expressing surface mOX40L vector or TART-1 parental cells, were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of C2515-40.
2. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added.
3. Cells were incubated on ice for 30 min., washed and then analyzed by Flow Cytometry.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2c6D294Affinity Purified
100ug4C (-20C Glycerol)Blue IceMouseRat
Rat cells stably overexpressing mouse CD134L (OX40L).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Recognizes mouse CD134L. Does not crossreact with human CD134L.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Murata, K., et al., J. Exp. Med. 191: 365 (2000).