Technical Data
C2548-90
CD163 (Hemoglobin/Haptoglobin Complex Receptor, M130 Antigen, Ber-Mac3, Ki-M8, SM4)
Description:
CD163 antigen is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 is restricted in its expression to the monocytic/macrophage lineage. It is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan’s cells.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Placenta

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.188Supernatant
SizeStorageShippingSourceHost
500ul-20°CProduct Type: Mab Isotype: IgG1 Clone No: 0.N.188 Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%. Purity: Tissue culture supernatant Immunogen: RecomHumanMouse
Concentration:
Not Determined
Immunogen:
Recombinant protein encoding domains 1 to 4 of the N-terminal region of human CD163. Cellular Localization: Cell membrane
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human CD163. May be useful in the localization of monocytes and most macrophage types as well as aiding further study into the function and expression of the antigen itself.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Droste, A., et al., Biochem. Biophys. Res. Commun. 256(1): 110-113 (1999). 2. Hogger, P., et al., J. Immunol. 161(4): 1883-1890 (1998).