Technical Data
cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)
Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues.

Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A.
Immunohistochemistry (formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval.
Western Blot: 2ug/ml for 2 hours at RT.
Optimal dilution determined by the researcher.

Positive Controls:
HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ug-20CBlue IceXenopusMouse
Xenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Recognizes Xenopus cdk1/p34cdc2 protein kinase at ~34kD. Does not crossreact with cdk2/p32 and other cyclin dependent kinases (cdks). Epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. Inhibits the activation of p43cdc2 kinase by cyclins and precipitates an active kinase. Species Crossreactivity: human, mouse, rat, guinea pig, woodchuck, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doussis-Anagnostopoulos, I.A., et al., Histopathology 24: 335 (1994).