Technical Data
C2549-50D
cdk1, p34cdc2 (CDC2, Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, MGC111195, p34 protein kinase, DKFZp686L20222)
Description:
Cdk1/p34cdc2 protein kinase is a catalytic subunit of Maturation Promoting Factor. p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Native, denatured): 2ug/mg protein lysate using Protein A.
Immunohistochemistry (formalin/paraffin, frozen): 2-4ug/ml for 30 minutes at RT. Paraffin sections require antigen retrieval.
Western Blot: 2ug/ml for 2 hours at RT.
Optimal dilution determined by the researcher.

Positive Controls:
HeLa or NIH3T3 cells; germinal center in a tonsil or reactive lymph node.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q305Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceXenopusMouse
Concentration:
~0.1mg/ml
Immunogen:
Xenopus p34cdc2. Cellular Localization: Nuclear with some cytoplasmic staining.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes Xenopus cdk1/p34cdc2 protein kinase at ~34kD. Does not crossreact with cdk2/p32 and other cyclin dependent kinases (cdks). Epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. Inhibits the activation of p43cdc2 kinase by cyclins and precipitates an active kinase. Species Crossreactivity: human, mouse, rat, guinea pig, woodchuck, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doussis-Anagnostopoulos, I.A., et al., Histopathology 24: 335 (1994).