Technical Data
cdc2 (Tyr15) Control Proteins
10 Blots
Molecular Biology Storage: -20CShipping: Blue Ice
Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161 (1-4). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of tyrosine 15 and threonine 14 (3,5). Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (3,4,6).

Suitable for use in Western Blotting. Other applications not tested.

Western Blots: As controls, we recommend using 10ul of GST-cdc2 negative control protein and 20ul of the positive control SK-N-MC cell extract.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
32, 34kD
Form: Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue or phenol red.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441470. (2) Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 16751685. (3) Watanabe, N. et al. (1995) EMBO J. 14, 18781891. (4) Galaktionov, K. et al. (1995) Genes Dev. 9, 10461058. (5) Hunter, T. (1995) Cell 80, 225236. (6) McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 7585.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.