Technical Data
C2549-50T
cdc2, Tyr15 (Cdk1, Cyclin Dependent Kinase 2)
Description:
During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:50
mmunocytochemistry: 1:500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG6k113Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Cdc2 without phosphorylation on Tyrosine 15. Does not detect Y15-phosphorylated Cdc2. Species Crossreactivity: mouse, rat. Species Sequence Homology: bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Norbury, C. and P. Nurse. Animal cell cycles and their control. Annu. Rev. Biochem. 61: 441–470 (1992).
2. Atherton-Fessler, S. et al. Mechanisms of p34cdc2 regulation. Mol. Cell. Biol. 13: 1675–1685 (1993).
3. Watanabe, N. et al. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. EMBO J. 14: 1878–1891 (1995).
4. Galaktionov, K. et al. Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev. 9: 1046–1058 (1995).