Technical Data
Cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)
The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated 2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).

Cellular Localization: Plasma membrane

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceYeastMouse
Full length protein (S. cerevisiae).
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Species Crossreactivity: Crossreacts with S. cerevisiae. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References:
1. Hall JR, et al. Mol Biol Cell. 18(9):3340-50, 2007. 2. Williams RS, et al. Proc Natl Acad Sci U S A. 94(1):142-7, 1997. 3. Yan Z, et al. Proc Natl Acad Sci U S A. 95(7):3603-8, 1998 4. Jiang W, et al. Proc Natl Acad Sci U S A. 96(11):6193-8, 1999.

This clone has been used in: Perkins G et al. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20:4836-45 (2001). Donovan S et al. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A 94:5611-6 (1997).