Technical Data
CDC25C, CT (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)
Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events. Cdc25C is activated via phosphorylation at Ser216. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis.

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Western Blot: 1:500
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
A synthetic peptide corresponding to residues in C-terminus of human Cdc25C.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, and 40% glycerol.
Recognizes human Cdc25C. Does not cross-react with other MPI phosphatase family members. Mol. Wt.: 54kD
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sadhu, K., et al. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc Natl Acad Sci U S A. 87: 5139–43 (1990).
2. Jessus, C. and R. Ozon. Function and regulation of cdc25 protein phosphate through mitosis and meiosis. Prog. Cell Cycle Res. 1: 215–228 (1995).
3. Peng, C.Y., et al. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277: 1501–1505 (1997).
4. Blasina, A., et al. A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr Biol. 9: 1–10 (1999).
5. Matsuoka, S., et al. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 282: 1893-7 (1998).