Technical Data
C2560-15E
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)
Description:
CDC25C is a member of Cdc25 phosphatase family which plays prominent role in the regulation of eukaryotic cell cycle progression. CDC25C activates the mitotic-promoting factor cyclin B1/cdc2 by dephosphorylating T14 and Y15 of cdc2/cyclin B and thus triggers G2/M transition. The protein acts as a converging point of several signal transduction cascades and links the mitotic activation of cdc2/cyclin B to the DNA replication and repair checkpoint. The enzymatic activity of CDC25C is low during interphase and increases sharply at the G2}M transition point. The protein is nuclear throughout interphase. Alternate splicing of its mRNA results in multiple transcript variants. Human CDC25C gene is localized in the chromosomal region 5q31.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human cdc25C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sartor, H. et al. Genomics. 13:911-912 (1992). 2. Zwicker, J. et al. Prog. Cell. Cycle. 1:91-99 (1995). 3. Schwindling, SL. et al. Nature. 23:4155-4165 (2004).