Technical Data
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)
CDC25C is a member of Cdc25 phosphatase family which plays prominent role in the regulation of eukaryotic cell cycle progression. CDC25C activates the mitotic-promoting factor cyclin B1/cdc2 by dephosphorylating T14 and Y15 of cdc2/cyclin B and thus triggers G2/M transition. The protein acts as a converging point of several signal transduction cascades and links the mitotic activation of cdc2/cyclin B to the DNA replication and repair checkpoint. The enzymatic activity of CDC25C is low during interphase and increases sharply at the G2}M transition point. The protein is nuclear throughout interphase. Alternate splicing of its mRNA results in multiple transcript variants. Human CDC25C gene is localized in the chromosomal region 5q31.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic non-phosphopeptide corresponding to human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recognizes human cdc25C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sartor, H. et al. Genomics. 13:911-912 (1992). 2. Zwicker, J. et al. Prog. Cell. Cycle. 1:91-99 (1995). 3. Schwindling, SL. et al. Nature. 23:4155-4165 (2004).