Technical Data
C2566-01K
CDCP1 (CUB Domain-containing Protein 1, Membrane Glycoprotein gp140, Subtractive Immunization M Plus HEp3-associated 135kD Protein, SIMA135, Transmembrane and Associated with Src Kinases, CD318, TRASK, UNQ2486/PRO5773) (PE)
Description:
CDCP1, also known as SIMA135, is a novel 140kD type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635aa extracellular region. The 148aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310aa residue form of CDCP1 also exists. The amino-terminal region of ~265aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow- derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption: 488nm

Emission: 565-605nm

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG112K58Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa33-666 from human CDCP1 expressed in NSO cell line.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes an epitope at aa370-666 of human CDCP1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.