		Technical Data
C2589-57E	CDX2 (Caudal Type Homeobox 2, Caudal-type Homeobox Protein 2, Homeobox Protein CDX-2, CDX3, CDX-3)

Description:
CDX2 is an intestine-specific transcription factor expressed early in intestinal development and may function in the regulation of proliferation and differentia- tion of intestinal epithelial cells. CDX2 is found predominantly on the surface of villi and crypts. Differential expression has been reported in some intestinal cancers. This antibody will help detect CDX2 in normal and uncharacterized tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:100 for1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Large bowel (nuclear staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	AMT28	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Fusion protein representing the 180 residue amino terminal portion of the human CDX2.

Purity:

Supernatant

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

Specificity:

Recognizes human CDX2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Bai Y Q, Yamamoto H, Akiyama Y, et al.. Ectopic expression of homeodomain protein CDX2 in intestinal metaplasia and carcinomas of the stomach. Cancer Letters. 176(1):47-55 (2002). 2.Hinoi T, Tani M, Lucas P C, et al.. Loss of CDX2 expression and microsatellite instability are prominent features of large cell minimally differentiated carcinomas of the colon. American Journal of Pathology. 159(6):2239-2248 (2001). 3.Sivagnanasundaram S, Islam I, Talbot I, et al.. The homeobox gene CDX2 in colorectal carcinoma: a genetic analysis. British Journal of Cancer. 84(2):218-225 (2001). 4.Bai Y-Q, Akiyama Y, Nagasaki H, et al.. Distinct expression of CDX2 and GATA4/5, development-related genes, in human gastric cancer cell lines. Molecular Carcinogenesis. 28:184-188 (2000). 5.Silberg D G, Swain G P, Suh E R, et al.. Cdx1 and cdx2 expression during intestinal development. Gastroenterology. 119(4):961-971 (2000).