Technical Data
C2589-79
CEACAM 6 (Carcinoembryonic Antigen (CEA)-Related Cell Adhesion Molecule 6, Non-specific Cross-reacting Antigen, Normal Cross-reacting Antigen, NCA, CD66c)
Description:
CEACAM6 is a GPI-anchored membrane protein with a single N domain, which shows structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like domains. CEA-related cell adhesion molecule 6 (CEACAM6, NCA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 90,000 which is strongly expressed on epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes (2,3). CEACAM6 expression is upregulated in many adenocarcinomas and leukemias. Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant-like A and B domain. This clone can be used to distinguish CEACAM6 from all other CEACAM and probably all pregnancy-specific glycoprotein molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members.

Applications:
Suitable for use in Flow Cytometry, ELISA, CELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1.2ug/10e6 cells
ELISA, CELISA: 1:100-1:200
Western Blot: 4ug/ml
Immunohistochemistry (Frozen): 1-2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15E506Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Tumor cell lines expressing CEACAM6.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes native human CEACAM6 expressed on transfected mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Daniel, S., et al., Int. J. Cancer 55: 303-310 (1993). 2. Gottlinger, H.G., et al., Cancer Res 48: 2198-2203 (1988). 3. Grunert, F., et al., CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. In Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York and London, pp. 1012-1025, (1996). 4. Hammarström, S., Semin. Cancer Biol. 9: 67-81 (1999). 5. Hanenberg, H., et al., Leukemia 12: 2127-2133 (1994). 6. Krop-Watorek, A., et al., Cancer Lett. 139: 15-22 (1999). 7. Muenzner, P., et al., J. Biol. Chem. 277: 7438-7446 (2002). 8. Muenzner, P., et al., Infect Immun. 68: 3601-3607 (2000). 9. Muenzner, P., et al., J. Biol. Chem. 276: 24,331-24,340 (2001). 10. Scholzel, S., et al., Am. J. Pathol. 156: 595-605 (2000). 11. Thompson, J., et al., Int. J. Cancer 55: 311-319 (1993). 12. Watt, S.M., et al., Blood. 98: 1469-1479 (2001). 13. Zimmermann, W., Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462 (2002).