Technical Data
C2615
CENP-A (Centromere Protein A)
Description:
The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex.

Applications:
Suitable for use in Immunoblot anaylsis. Other applications have not been tested.

Recommended Dilution:
Immunoblot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. .KLH conjugated
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 5mg/ml BSA, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zeitlin, S., et al., J. Cell Sci. 114: 653-661, 2001.